Sample Reception and Entry
The DNA ploidy sample is received and entered into the MOITC online system to ensure accurate tracking and documentation.
Slide Preparation
The sample undergoes a process to prepare slides using a specialized slick solution, which is crucial for the subsequent analysis.
Böhm-Springer Fixative Treatment
The prepared slides are treated with Böhm-Springer fixative at 35 degrees Celsius for 3 hours. This step ensures the preservation of cellular structures for accurate analysis.
Hydrochloric Acid (HCl) Treatment
The slides are then exposed to 5N HCl for 25 minutes. This step is critical for breaking down cellular proteins, allowing for better visualization of DNA.
Dehydration with Ethanol Solution
The slides are dehydrated using a graded ethanol solution, which helps to remove water and prepare the cells for staining.
Staining Process
The cells on the slides are stained with eosin and other dyes. This staining is vital for differentiating between normal and abnormal cells.
Slide Scanning, Analysis and Reporting
Finally, the prepared and stained slides are scanned using advanced imaging technology. The digital images are then analyzed for DNA content, helping to identify potentially malignant cells.